EAN: 9781493923977

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This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes the second half of the book examines four main types of approaches: comparative genomics supervised machine learning constraint-based methods and corrections of transposon mutagenesis data as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format chapters include an introduction to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.

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