EAN: 9781617798696

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Population genomics is a recently emerged discipline which aims at understanding how evolutionary processes influence genetic variation across genomes. Today in the era of cheaper next-generation sequencing it is no longer as daunting to obtain whole genome data for any species of interest and population genomics is now conceivable in a wide range of fields from medicine and pharmacology to ecology and evolutionary biology. However because of the lack of reference genome and of enough a priori data on the polymorphism population genomics analyses of populations will still involve higher constraints for researchers working on non-model organisms as regards the choice of the genotyping sequencing technique or that of the analysis methods. Therefore Data Production and Analysis in Population Genomics purposely puts emphasis on protocols and methods that are applicable to species where genomic resources are still scarce. It is divided into three convenient sections each one tackling one of the main challenges facing scientists setting up a population genomics study. The first section helps devising a sampling and or experimental design suitable to address the biological question of interest. The second section addresses how to implement the best genotyping or sequencing method to obtain the required data given the time and cost constraints as well as the other genetic resources already available Finally the last section is about making the most of the (generally huge) dataset produced by using appropriate analysis methods in order to reach a biologically relevant conclusion. Written in the successful Methods in Molecular Biology(TM) series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible protocols advice on methodology and implementation and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Data Production and Analysis in Population Genomics serves a wide readership by providing guidelines to help choose and implement the best experimental or analytical strategy for a given purpose.

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19.03.2025 um 11:37 Uhr

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